Home / Research & Development / Sequencing Based Typing

Research & Development

Sequencing Based Typing

The HLAssure™ SBT Kits are for determining HLA alleles using PCR techniques with DNA sequencing. Group and Locus specific primer pairs are designed to amplify target sequences which are specific to a single allele or group of alleles. The SBT method is based on the principle that primers will target conserved HLA sequences resulting in amplified products under controlled PCR conditions.

The presence of amplified DNA fragment is a positive indication of the existence of allele and can be further identified via DNA sequencing. DNA Sequencing is the gold standard of molecular diagnostics and can effectively provide high resolution results for HLA Typing.